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Santa Cruz Biotechnology
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Proteintech
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Synaptic Systems
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Synaptic Systems
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Synaptic Systems
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Synaptic Systems
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Synaptic Systems
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Chemie GmbH
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ABclonal Biotechnology
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Synaptic Systems
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Gallus BioPharmaceuticals
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Image Search Results
Journal: ACS chemical neuroscience
Article Title: Chronic Social Isolation Stress during Peri-Adolescence Alters Presynaptic Dopamine Terminal Dynamics via Augmentation in Accumbal Dopamine Availability
doi: 10.1021/acschemneuro.8b00360
Figure Lengend Snippet: Assessment of several terminal protein expression levels. Relative protein expression levels of (A) VMAT2 (n = 7 in both groups), (B) Synaptigyrin-3 (aGH: n = 6; aSI: n = 5), (C) Syntaxin-1 (aGH: n = 7; aSI: n = 6), and (D) Munc13-3 (aGH: n = 8; aSI: n = 8) were measured. None of these proteins had different expression levels in aGH and aSI rats. (insets) The representative Western blot images, with the respective protein and actin for comparison. Group housed, aGH, blue; Socially isolated, aSI, red.
Article Snippet: Subsequently, blots were incubated with agitation for 2 h at room temperature in TBS-T/5% bovine serum albumin (05470; Sigma-Aldrich) solution containing the following primary antibody concentrations: VAMT2 (1:2000; AB1598P; Millipore Sigma); Synaptogyrin-3 (1:1000; ab106460; abcam);
Techniques: Expressing, Western Blot, Isolation
Journal: Journal of Diabetes Investigation
Article Title: SNARE ‐binding protein synaptosomal‐associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes
doi: 10.1111/jdi.13912
Figure Lengend Snippet: Synaptosomal‐associated protein of 29 kDa (SNAP29)–syntaxins interaction. (a) 3T3‐L1 adipocytes were not serum‐starved. The SNAP29–syntaxins interactions were determined by immunoprecipitation using anti‐SANP29, anti‐syntaxin4, anti‐syntaxin6 or non‐immune control rabbit antibodies. (b) 3T3‐L1 adipocytes were serum‐starved for 2 h and treated with 100 nmol/L insulin for 0, 5 or 20 min. The SNAP29–syntaxin6 interaction was determined by immunoprecipitation using anti‐synatxin6 antibody, and the precipitates and start lysates were immunoblotted with anti‐SNAP29, anti‐syntaxin6, antiphospho‐Ser473 protein kinase B (Akt) and anti‐Akt antibodies. The graph shows the results of quantitative analysis for each band by Image J software.
Article Snippet: The following antibodies were used: anti‐GLUT4, anti‐GFP, anti‐GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti‐HA (HA‐7; Sigma Aldrich, St. Louis, MO, USA); anti‐SNAP29,
Techniques: Immunoprecipitation, Control, Software